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Cell Tech FLNADH 100 說明書

更新時間:2017-08-02      點擊次數:3890

世界*實驗材料供應商 Cell Technology正式授權上海起發為其中國代理, Cell Technology在一直是行業的*,一直為廣大科研客戶提供zui為的產品和服務,上海起發一直秉承為中國科研客戶帶來的產品,的服務,簽約 Cell Technology就是為了給廣大科研客戶帶來更加完善的產品和服務,您的滿意將是我們zui大的收獲

Cell Technology中國代理, Cell Technology上海代理, Cell Technology北京代理,Cell Technology廣東代理, Cell Technology江蘇代理Cell Technology湖北代理,Cell Technology天津,Cell Technology黑龍江代理,Cell Technology內蒙古代理,Cell Technology吉林代理,Cell Technology福建代理, Cell Technology江蘇代理, Cell Technology浙江代理, Cell Technology四川代理,

 

Cell Technology成立于1998年,致力于為研究院、生物技術公司及制藥企業的科研人員研發生產*的細胞功能研究用試劑與試劑盒。這些檢測試劑與試劑盒可以實現不破壞細胞形態和結構的情況下原位檢測細胞成分,研究細胞內發生的特定事件.Cell Technology發布了通過測定胞內活性caspase及線粒體膜電位研究細胞凋亡的一系列試劑。隨著公司研發團隊的持續努力,我們又推出了一系列高特異性的caspase檢測試劑,以及cathepsin檢測的試劑和ELISA試劑盒。目前Cell Technology提供的檢測試劑和ELISA試劑盒涵蓋Caspase、NOS、ROS、線粒體膜電位、信號通路和蛋白磷酸化等多個研究領域,產品倍各大高校、研究機構、生物技術企業和制藥企業采用。

 

Cell Technology主要產品簡介

1、Fluoro Cholesterol:高靈敏、一步檢測生物樣本中總膽固醇含量,可用于細胞代謝與藥物作用的研究。

2、Fluoro NADP:檢測組織或細胞提取液內NADP/NADPH含量,與NAD/NADH檢測無交叉,可用于細胞死亡、抗氧化/氧化應激、能量代謝及線粒體功能研究。

3、Fluoro Sarcosine:定量檢測細胞、血清及組織提取物中的肌氨酸含量。

4、Fluoro IndoBlu:定量檢測細胞中IndoBlu的含量,適用于細胞增殖研究。

5、Fluoro Lactate:定量檢測生物樣本中 L-lactate的含量。

6、Fluoro Phosphate:定量檢測生物樣本中的磷含量,靈敏度可達200nM。

 

 

 

 

 

 

  • 產品編號:FLNADH 100-2
  • 產品名稱:Fluoro NAD™ Fluorescent NAD/NADH Detection Kit - Detection of NAD/NADH in tissue extracts /cell samples/熒光NAD/NADH檢測試劑盒
  • 產品規格:100 Tests
Fluoro NAD™ NEW!
Fluorescent NAD/NADH Detection Kit - Detection of NAD/NADH in tissue extracts /cell samples

 
Key Benefits:
  • Detection of NAD/NADH activity in cells or tissue extracts
  • Detection of NAD/NADH levels in apoptosis, metabolism, proliferation, DNA repair, senescence, endocrine signaling and life span.
  • NAD/NADH detection in Bacterial, fungal and plant cells.
  • Fluorescent 96 well Plate Reader Readout (excitation: 530-570nm and emission at 590-600nm)
Introduction

The role of pyridine nucleotides (NAD/NADH) metabolism in health, is of continual and increased interest. A growing amount of evidence supports the fact that NAD metabolism regulates important biological effect including life span. NAD, through poly-ADP-ribosyl polymerase (PARP), mono-ADP-ribosyltransferase (ARTs) and recently characterized sirtuin enzymes, exerts potential biological effects. These enzymes modify proteins to regulate their function via ADP-ribosylation or deacetylation and are involved in several pathways including apoptosis, DNA repair, senescence and endocrine signaling. This suggests that either the enzymes or NAD could be an important therapeutical target 1.

 
Assay Principle
The Fluoro NAD/NADH detection kit utilizes a non-fluorescent detection reagent, which is oxidized in the presence NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction. The enzyme reaction specifically reacts with NAD/NADH and not with NADP/NADPH.

Reaction:

1. NADH + non-fluorescent detection reagent+ electron coupler -----> fluorescent 
                                                                                                                    analog + NAD

  2. NAD + enzyme coupled reaction -------> NADH (then proceeds to reaction 1).

  excitation: 530-570nm and emission at 590-600nm

 

References
Anthony A. Sauve NAD+ and Vitamin B3: From metabolism to therapies
J. Pharmacol. Exp. Ther. 2007 : jpet.107.120758v1 
Kit contents (for 100 assays)
  1. Enzyme Mix   ..................................................Part# 6021 - Store at -20C
  2. NADH Detection Reagent .............................Part# 4018 - Store at -20C
  3. NADH Standard .............................................Part# 7013 - Store at -20C
  4. NAD/NADH Reaction Buffer  ........................Part# 3044 - Store at +2-8 C
  5. NAD/NADH Lysis Buffer ..............................Part# 3045 - Store at +2-8 C
  6. NAD Extraction Buffer ..................................Part# 3046 - Store at +2-8 C
  7. NADH Extraction Buffer ................................Part# 3047 - Store at +2-8 C
  8. NADH Standard Diluent ................................Part# 3051 - Store at +2-8 C
The following kits are available:
Catalog # Size Price (RMB)
FLNADH 100-2100 Tests5250.00

 

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